Latest News & Information
Come see our presentation at HUPO in Dublin Ireland with Bruker: "microDIA acquisition combined to a novel software tool enables direct identification of peptides in pseudo-SRM MS/MS spectra from sequence databases."
An integrated, high-throughput, multi-omics platform enables data-driven construction of cellular responses and reveals global drug mechanisms of action
January 14, 2017
Evaluation of Stem Cell-Derived Red Blood Cells as a Transfusion Product Using a Novel Animal Model
December 13, 2016
microDIA Product Launch
December 1, 2016
Stay tuned for more details!
Join Us at ASMS in San Antonio on May 7th at 7PM Casa Rio
May 7, 2016
For more information on ASMS Trade Shows click here.
News Flash: Our manuscript on traditional DIA, PMID 26757066, was accepted from work with collaborators at the University of Cincinnati
Systematic evaluation of data-independent acquisition for sensitive and reproducible proteomics—a prototype design for a single injection assay
December 1, 2015
DIA Increased Ability to Quantify Peptides Across Samples
October 27, 2015
A major advantage of SWATH-DIA is a superior ability to match peptides identified to extracted fragment-ion chromatograms relative to the percentage of identified peptides in shotgun analyses that can be matched to a corresponding precursor-ion chromatogram. This enhanced ability to detect fragment-ion chromatograms by DIA-SWATH is due to better signal-to-noise in MS/MS scans relative to survey scans. A transparent readout of the percentage of identified peptides that can be matched to fragment-ion chromatograms is provided in Protalizer’s quality metrics reports that are automatically generated by the program with each analysis.
Increasing the Amount of Informational Content Obtained From Comparative Proteomic Studies Through DIA Assays With Protalizer
February 26, 2015
DIA assays offer the ability to obtain more confidence in protein changes detected across biological conditions compared via fragment ion chromatograms showing a series of fragment ions assigned to each peptide quantified, rather than having only the matched MS1 precursor intensity peak for quantification.
One limitation that currently exists is having to manually extract peptide fragment ion chromatograms in Skyline or with instrument-vendor viewers such as PeakView with AB Sciex Triple-TOF instruments that is time prohibitive in large scale DIA studies where thousands of peptides and proteins are quantified.
We developed the Protalizer DIA software package to provide a solution to this by automatically generating archives of fragment ions showing every peptide quantified along with the most consistent endogenous peptide identified across all the samples compared with similar elution time as a control for instrument and sample preparation variability. These automatically generated fragment ion chromatograms are organized by protein name and allow any users in your organization or collaborators that are not mass spectrometry experts to use for reviewing and verifying results, not to mention saving mass spectrometry operators countless hours that have to be spent to extract even as few as 50 peptides across samples compared.
One limitation that currently exists is having to manually extract peptide fragment ion chromatograms in Skyline or with instrument-vendor viewers such as PeakView with AB Sciex Triple-TOF instruments that is time prohibitive in large scale DIA studies where thousands of peptides and proteins are quantified.
We developed the Protalizer DIA software package to provide a solution to this by automatically generating archives of fragment ions showing every peptide quantified along with the most consistent endogenous peptide identified across all the samples compared with similar elution time as a control for instrument and sample preparation variability. These automatically generated fragment ion chromatograms are organized by protein name and allow any users in your organization or collaborators that are not mass spectrometry experts to use for reviewing and verifying results, not to mention saving mass spectrometry operators countless hours that have to be spent to extract even as few as 50 peptides across samples compared.
Vulcan Analytical Launches DIA Software Program
September 03, 2014
Vulcan Analytical at ASMS in St. Louis May 31st - June 4th 2015. Please contact us at info@vulcan.ms to setup an in-person meeting.
Why Hasn’t Every Biological Lab Done a Mass Spectrometry Based Comparative Proteomics Study to Determine Proteins That Are Differentially Regulated?
March 10, 2014
First, most labs want reliable quantification of proteins but cannot afford to purchase stable isotope technologies in the current funding scenario.
Second, many researchers have previously done proteomics studies, or know a colleague that has, and not been able to verify the quantitative differences reported to them by independent methods such as western blotting.
We developed Protalizer to solve these limitations by creating a different proprietary approach to accurately normalize relative peptide concentrations that outperforms existing label-free approaches such as total-ion-current corrections. In addition, during the development of Protalizer the results obtained showing altered protein concentrations in experimental groups were validated and steps are implemented in the program to eliminate quantification results that are unreliable without substantially sacrificing sensitivity.
Second, many researchers have previously done proteomics studies, or know a colleague that has, and not been able to verify the quantitative differences reported to them by independent methods such as western blotting.
We developed Protalizer to solve these limitations by creating a different proprietary approach to accurately normalize relative peptide concentrations that outperforms existing label-free approaches such as total-ion-current corrections. In addition, during the development of Protalizer the results obtained showing altered protein concentrations in experimental groups were validated and steps are implemented in the program to eliminate quantification results that are unreliable without substantially sacrificing sensitivity.
Vulcan Analytical at American Society for Mass Spectrometry
February 18, 2014
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Vulcan Analytical is sponsoring booth #65 at the 62nd ASMS conference on Mass Spectrometry and Allied Topics, June 15-19, 2014 in Baltimore. We look forward to seeing you there! To set up a meeting with us at the 2014 ASMS conference please contact us.
62nd ASMS Conference on Mass Spectrometry and Allied Topics June 15 - 19, 2014, Baltimore Convention Center, Baltimore, MD |
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Free Trial Access to Protalizer is Now Available
February 14, 2014
Vulcan Analytical is proud to announce availability of free trial access to Protalizer, a mass spectrometry based comparative proteomics solution.
UAB Biotech Startup Wins $22,000 in Launchpad Competition
April 18, 2012
Vulcan Analytical wins $22,000 in a prestigious business plan competition named Alabama Launchpad on April 17th 2012. EDPA-sponsored contest considered several start-ups for this honor, with Vulcan Analytical taking one of the four prizes.
Read Full Article Here
Read Full Article Here
